Application

Research CategorySignaling

Immunohistochemistry Analysis: A 1:50 dilution from a representative detected FABP2 in human small intestine and rat colon tissue sections.

Western Blotting Analysis: A 1:1,000 dilution from a representative detected FABP2 in 10 µg of human small intestine tissue lysate.

Detect FABP2 using this mouse monoclonal Anti-FABP2 Antibody, clone 4F6.1, Cat. No. MABS1694, validated for use in Immunohistochemistry (Paraffin) and Western Blotting.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Fatty acid-binding protein, intestinal (UniProt P12104; also known as Fatty acid-binding protein 2, I-FABP, Intestinal-type fatty acid-binding protein) is encoded by the FABP2 (also known as FABPI) gene (Gene ID 2169) in human. The intracellular lipid-binding protein (iLBP) multigene family includes 12 fatty acid-binding protein (FABPs), 4 retinol-binding proteins (RBPs) and 2 cellular retinoic acid-binding proteins (CRABPs). FABPs are 14-16 kDa cytosolic proteins that bind fatty acids (FAs) and other hydrophobic ligands, such as eicosanoids and retinoids. FABPs are either numbered by the Hertzel and Bernlohr nomenclature to indicate their chronological order of discovery or named according to the tissue where each FABP was first isolated. In addition to transporting and sequestering long-chain FAs, eicosanoids, bile salts, and other hydrophobic ligands for storage and metabolism, FABPs are long recognized for their role in gene transcription regulation via lipid-mediated activation of nuclear hormone receptors (NHRs). FABP1 and FABP2, for example, are shown to bind a range of PPAR agonists, including long chain fatty acids (LCFA) and drugs. FABPs play differential and ligand-specific roles in NHR activation, and the patterns of drug interaction with intracellular binding proteins such as FABPs may govern tissue-specific actions of drugs that target NHRs.

Immunogen

GST-tagged recombinant human FABP2.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Purified mouse IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Protein G purified.

Format: Purified

Quality

Evaluated by Western Blotting in mouse small intestine tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected FABP2 in 10 µg of mouse small intestine tissue lysate.

Specificity

Clone 4F6.1 targets an epitope conserved among human, mouse, and rat species.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~15 kDa observed. 15.11 kDa (human), 14.99 kDa (mouse & rat) calculated (Met1 removed). Uncharacterized bands may be observed in some lysate(s).